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If you want to return to your practical later, using your own Linux or Mac OS X computer, you can download the demo version of CYANA from here.
Hint: More information on the CYANA commands etc. is in the CYANA 3.0 Reference Manual.
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Resonance assignment within cyana CYANA is done using the module FLYA.
In the most general sense, there are two type of experiments used for protein resonance assignments. Through bond , TOSCY kind of experiments (like TOSCY, COSY) and through space NOESY type of experiments (like NOESY). Each of these two experiments carries distinct information that help the resonance assignment. The HSQC , or HMQC or TROSY elements of these experiments merely help the resolution, by allowing the separation of resonances according to spin types (1H, 13C, 15N) into additional dimensions.
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