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It used to be common to only simply classify the experimental NOE intensities to strong, medium and weak, and assign corresponding distance ranges of around 2 Angsrom for the strongest and 5.5 to the weakest.
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Here we use a technique, which would be a starting point for more accurate methods. It uses spectra recorded for different mixing times. As the NOESY spectrum of a protein can take days to record, recording series of them is a large investment. In that the NOE crosspeak volume (intensity) is divided by a geometric mean of the corresponding diagonal volumes. The slope is a better approximation of the crossrelaxation rate than if this "linearization" or "normalization" was not done. In the case of two isolated nuclei, such buildup curve is approximately linear over longer mixing times.
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