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In the most general sense, there are two type of experiments used for protein resonance assignments. Through bond kind of experiments (like TOSCY, COSY) and through space type of experiments (like NOESY). Each of these two experiments carries distinct information that help the resonance assignment. The HSQC or HMQC elements of these experiments merely help the resolution, by allowing the separation of resonances according to spin types (1H, 13C, 15N) into additional dimensions.
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Spectra are processed and referenced relative to each other. Peak lists in XEASY format are prepared by automatic peak picking with a visualization program such as CcpNmr Analysis, NMRdraw or NMRview and saved as XXX.peaks, where XXX denotes the name of the xeasy XEASY peak list file. Then they are cleaned (unnecessary water and noise peaks removed).
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